Plasmid pCMV·SPORT-βgal
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Invitrogen™

Plasmid pCMV·SPORT-βgal

Plasmid pCMV·SPORT-bgal is used as a positive control formonitoring expression in eukaryotic cells. The plasmid contains thereporter gene b-galactosidase (b-gal)Read more
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Catalog number 10586014
Price (USD)
772.00
Each
Add to cart
Price (USD)
772.00
Each
Add to cart
Plasmid pCMV·SPORT-bgal is used as a positive control for
monitoring expression in eukaryotic cells. The plasmid contains the
reporter gene b-galactosidase (b-gal) from E. coli cloned as a Not I
fragment into plasmid pCMV·SPORT1. The plasmid also contains
a CMV promoter upstream of the b-gal gene, followed by the SV40
t-intron and polyadenylation signal. The b-lactamase gene allows
selection for ampicillin resistance in E. coli.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypePlasmid
Reporter GeneBeta-Gal (lacZ)
Selection Agent (Eukaryotic)None
Shipping ConditionApproved for shipment on Wet or Dry Ice
Protein TagUntagged
Quantity25 μg
VectorpCMV
For Use With (Application)Reporter Assays, Constitutive Expression
Unit SizeEach
Contents & Storage
Store at +4°C. Store at -20°C long term.

Frequently asked questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.