Expi293F™ Cells (cGMP banked)
For product ordering information, please contact us at outlicensing@thermofisher.com.
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Gibco™

Expi293F™ Cells (cGMP banked)

Expi293F Cells are derived from the human 293 cell line, and are a core component of the Expi293 Expression System.Read more
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Catalog number 100044202
Price (USD)
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Expi293F Cells are derived from the human 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F Cells are highly transfectable and achieve higher protein yields per cell compared to standard 293 cell lines resulting in transient protein expression yields of up to one gram per liter.

• For product ordering information, please contact us at outlicensing@thermofisher.com
• cGMP-banked suspension culture cells, pre-adapted to Expi293 Expression Medium
• Derived from the same Master Seed Bank as our standard Expi293F Cells (Cat No. A14527)
• Fully documented cGMP-banked cell line
• A component of the fully integrated, animal origin-free Expi293 Expression System for applications requiring cGMP-banked cells
• A Trial Licensing Program allows you or your CMO/CRO provider to purchase one vial of cells for research use with a simple, one-time payment, enabling full evaluation before committing to a commercial license
• Flexible commercial licensing options without royalties

Frozen cells are supplied in a vial containing 1.1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. Cells should be thawed directly into Expi293 Expression Medium.

Fully documented cGMP cell line
After execution and payment for a Commercial Production License, a Cell Line Documentation Package (>200 pages and specific to the lot of cells purchased) is provided that includes the entire lineage history of the cells starting at receipt of the initial vial at Thermo Fisher Scientific through delivery to the purchaser, as well as all testing reports.

Commercial licensing that is simple, flexible, and without royalties
Our Trial Licensing Program allows you or your CMO/CRO provider to obtain cGMP-banked Expi293F Cells for research use only with a one-time payment. As you move towards commercial use, a flexible Commercial Production or Service License structure is available without the burden of royalties, keeping it simple as a one-time payment. For more information regarding our Trial Licensing Program and commercial licensing, email us at outlicensing@thermofisher.com.
Specifications
Cell LineExpi293F
Culture TypeSuspension Cell Culture
Product LineExpi293™
Product TypeExpi293F Cells
Quantity1 vial
Shipping ConditionLN2 Dry Shipper
Unit SizeEach
Contents & Storage
1.1 mL (1 x 107 cells/mL), store in liquid nitrogen

Frequently asked questions (FAQs)

Do I need to remove Anti-Clumping Agent and Pluronic F-68 from the medium while growing Expi293 cells? How about during transfection of these cells?

You can have Anti-Clumping Agent and Pluronic F-68 in the medium while growing Expi293 cells. You need to remove Anti-Clumping Agent at least 2 passages prior to transfection of Expi293 cells because it interferes with transfection. You can add it back to the medium after transfection. Pluoronic F-68 can be present during transfection as it does not interfere with transfection.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I learn more about commercial licensing of Expi293F Cells (cGMP banked)?

To inquire about an Evaluation License, or Commercial Production License, or Service License, please email: outlicensing@thermofisher.com.

What is the term of the license agreement for Expi293F Cells (cGMP banked)?

As long as the license is not in breach, commercial use rights are granted in to perpetuity for the Commercial Production License. The term of the Commercial Service License is negotiated between the parties.

For the Expi293F Cells (cGMP banked), is there a pull-through royalty on final products or an annual maintenance fee?

Only a one-time license fee is required for a Commercial Production License, and it covers use during the clinical trial process and continues through the commercialization of one molecule.

Can I test the Expi293F Cells (cGMP banked) before committing to a Commercial License?

Yes. We offer an Evaluation Licensing Program, which allows you to purchase 1 vial of Expi293-F Cells (cGMP banked) for a one-time payment. A Limited Use Label License (“LULL”) accompanies each Evaluation License purchased from Life Technologies Corporation (“LIFE”) by the LIFE customer (“Customer”). The rights granted to a Customer with the purchase of an Evaluation License in the LULL include the following:

- Internal research use by Customer of such purchased Cell Line and the resulting molecules produced by such Cell Line

- Transfer of such Cell Line by Customer to a service provider (e.g., contract manufacturing organization (“CMO”) or contract research organization (“CRO”)) for Cell Line development and/or protein production solely for research and solely on behalf of and for the benefit of the Customer who purchased such Cell Line