eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
Invitrogen™

eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors andRead more
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Catalog number 00-5523-00
Price (USD)
231.00
Each
Add to cart
Price (USD)
231.00
Each
Add to cart
The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

For your convenience, we have provided these protocols for preparing your samples for intracellular antigen detection on a flow cytometer.

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 kit
Reagent TypeFixation Diluent, Fixation Reagent, Permeabilization Diluent, Permeabilization Reagent
Product LineeBioscience™
Product TypeStaining Buffer
Unit SizeEach

Frequently asked questions (FAQs)

Can I use the Foxp3 / Transcription Factor Staining Buffer Set to process samples for imaging?

The Foxp3 / Transcription Factor Staining Buffer Set was optimized for processing samples for flow cytometry; it may work for samples for imaging analysis, but we have not tested this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I get the identity/formulation for the individual components in the Foxp3 / Transcription Factor Staining Buffer Set?

The fixative is formaldehyde, the identity and formulation of other kit components are proprietary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is shipped with the Foxp3 / Transcription Factor Staining Buffer Set and what is their shelf life and storage conditions?

The Foxp3 / Transcription Factor Staining Buffer Set includes 3 bottles:
- Fixation/Permeabilization Concentrate (4X) (30 mL)
- Fixation/Permeabilization Diluent (100 mL)
- Permeabilization Buffer (100 mL)
They should be stored at 2-8 degrees C and should be used within 6 months.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) with the Blimp1-PE antibody from another company?

Yes, the eBioscience Foxp3/Transcription Factor Staining Buffer Set will allow any antibody to reach an intra-nuclear target to stain cells, including antibodies from other vendors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can eVolve-conjugated antibodies be used in combination with intracellular staining?

Yes! eVolve conjugates were designed to be compatible with eBioscience's intracellular staining buffers including the Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) and the Intracellular Fixation and Permeabilization Buffer Set (Cat. No. 88-8824-00).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set FAQs