eBioscience™ Brefeldin A Solution (1000X)
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Invitrogen™

eBioscience™ Brefeldin A Solution (1000X)

Brefeldin A is an inhibitor of intracellular protein transport. Incubation of cells in culture with Brefeldin A leads to blockadeRead more
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Catalog number 00-4506-51
Price (USD)
101.00
Each
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Price (USD)
101.00
Each
Add to cart
Brefeldin A is an inhibitor of intracellular protein transport. Incubation of cells in culture with Brefeldin A leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER). Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines. Brefeldin A is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Monensin in their specific assay system.


Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 mL
Product LineeBioscience™
Physical FormLiquid
Product TypeInhibitor
Unit SizeEach

Frequently asked questions (FAQs)

What do Monensin and Brefeldin A do?

These are critical chemicals for inclusion in cell cultures during cell activation prior to analysis by flow cytometry for the presence of intracellular chemokines and cytokines. Monensin (Cat. No. 00-4505-51) and Brefeldin A (Cat. No. 00-4506-51) are protein transport inhibitors that block secretion of proteins by cells via the Golgi apparatus, thereby causing an accumulation of cytokines at the endoplasmic reticulum or Golgi. Cells are often incubated with either of these two chemicals during cell activation in order to promote the accumulation of detectable protein levels within the cell. These are often essential reagents that allow intracellular detection of proteins that would otherwise be too low in abundance to detect. Monensin is known to block the transport from the medial to the trans cisternae of the Golgi stack. Brefeldin A has been reported to block protein transport from the ER. Specific information about the use of Monensin and Brefeldin A can be found on their respective Technical Data Sheets.

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