eBioscience™ Flow Cytometry Staining Buffer
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Invitrogen™

eBioscience™ Flow Cytometry Staining Buffer

Our Flow Cytometry Staining Buffer is designed for use in immunofluorescent staining protocols of cells in suspension. This Flow CytometryRead more
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Catalog NumberQuantity
00-4222-26600 mL
00-4222-57200 mL
Catalog number 00-4222-26
Price (USD)
164.00
Each
Add to cart
Quantity:
600 mL
Price (USD)
164.00
Each
Add to cart
Our Flow Cytometry Staining Buffer is designed for use in immunofluorescent staining protocols of cells in suspension. This Flow Cytometry Staining Buffer is a buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. This buffer can be used for antibody and cell dilution steps, as well as all the wash steps required for the surface staining and flow cytometric analysis. This buffer contains animal serum proteins to help minimizing non-specific binding of antibodies.

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity600 mL
Product LineeBioscience™
Product TypeStaining Buffer
Unit SizeEach

Frequently asked questions (FAQs)

Is it necessary to wash OneComp eBeads after staining?

One wash with 2 mL of Flow Cytometry Staining Buffer (Cat. No. 00-4222-26, 00-4222-57) is recommended for ideal staining of OneComp eBeads. However, it may be possible in some cases to use unwashed OneComp eBeads, if the antibody staining concentration is low enough. In testing, it appears that concentrations starting below 0.06 µg per test may provide appropriate compensation values. Usage in this way should be confirmed in the individual antibody of interest.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can eFluor Organic fluorochromes and eVolve QDots be used in combination with other organic fluorochromes?

Yes, both the eFluor Organic Dyes and eVolve QDots are fully compatible with conventional fluorochromes and can be used in the same manner as the fluorochromes that they are replacing. We recommend the Flow Cytometry Staining Buffer (Cat. No. 00-4222-26, 00-4222-57) as stated in the Staining Cell Surface Antigens for Flow Cytometry Protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.