Brilliant Ultra Violet 395 excitation shown as dotted line and emission shown as solid violet histogram
3355379/28347399(in buffer) 5
flow cytometry

Invitrogen Brilliant Ultra Violet™ 395 (BUV395) dye is excited by the 350 nm UV laser and emits at 395 nm. This dye has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BUV395 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Ultra Violet™ 395 primary antibodies

Experimental data using Brilliant Ultra Violet™ 395

Spectral signature of Brilliant Ultra Violet™ 395 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 395 were used for analysis.

 

Dot plot of IFN-gamma BUV395 vs CD4 FITC in unstimulated and stimulated cells

Intracellular staining of human peripheral blood cells using Brilliant Ultra Violet™ 395. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (right). Cells were then stained intracellularly, using Intracellular Fixation & Permeabilization Buffer Set, CD4 Monoclonal Antibody (RPA-T4), FITC and IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 395. Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

Dot plot of samples stained with IgG1 control vs CD19-FITC and CD3-BUV395 vs CD19-FITC

Cell surface staining of human peripheral blood cells using Brilliant Ultra Violet™ 395. Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 395 (left) or CD3 Monoclonal Antibody, Brilliant Ultra Violet™ 395 (right). Cells in the lymphocyte gate were used for analysis.

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.