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Isolate Plasmid DNA for Every Application
Our Invitrogen plasmid isolation kits offer options to eliminate the challenges raised by multi sample processing. We design our plasmid DNA extraction products to isolate plasmid DNA at the purity and scale you need. Choose from our wide range of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities. Learn about how to purify plasmid DNA with Invitrogen PureLink and Thermo Scientific GeneJET extraction kits with our plasmid isolation protocol videos.
Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of protein expression workflow. Learn more about how you can obtain transfection grade plasmid and use them with Gibco ExpiCHO and Expi293 transient mammalian expression systems.
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Which plasmid DNA isolation and purification kit is right for you?
| Rapid isolation of molecular biology quality | Gold standard for ultrapure, high quality | Enhanced purity for sensitive applications | ||
|---|---|---|---|---|
| Purity grade | Molecular | Transfection | Advanced transfection | |
| Endotoxin | Standard (>10 EU/µg) | Low endotoxin (0.1–1 EU/µg) | Endotoxin-free (< 0.1 EU/µg) | |
| Plasmid DNA purification kit |  GeneJET |  PureLink HiPure |  PureLink Fast |  PureLinkExpi Endotoxin-Free |
| Total protocol time | 15–60 min | 30–120 min | 30 min | 90–120 min |
| Prep size | Mini–Maxi | Mini–Giga | Midi, Maxi | Maxi, Mega, Giga |
| Yield up to | 20 µg–1 mg | 20 µg–15 mg | 0.4 mg (midi prep) or 1.5 mg (maxi prep) | 1.5–15 mg |
| Technology | Silica membrane | Anion exchange | Advanced silica | Anion exchange membrane |
| Applications | Not sensitive
| Sensitive
| Very sensitive
| |
| Order now | Order now | Order now | Order now | |
Plasmid DNA Preparation Sizes
Extract Plasmid DNA with PureLink and GeneJet
How to purify endotoxin-free plasmid DNA
Watch this video to learn how to purify plasmid DNA using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit. This video shows you how to isolate and purify high-quality, endotoxin-free plasmid DNA for sensitive applications like primary cell transfection.
How to purify molecular-grade plasmid DNA
This video shows you how to purify molecular-grade plasmid DNA in 15 minutes using our GeneJET Plasmid Miniprep Kit. Molecular-grade plasmid DNA is suitable for cloning, nucleic acid labeling, PCR, DNA sequencing, and transformation.
How to purify plasmid DNA using our megaprep kit
Isolate transfection-grade plasmid DNA in as little as 90 minutes with the Invitrogen PureLink HiPure Expi Plasmid Megaprep Kit. This innovative proprietary anion-exchange resin in these kits combines large capacity with fast flow rate, high resolution, and efficiency.
How to purify transfection grade plasmid DNA
Learn how to purify transfection grade plasmid DNA with the Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit. Transfection grade plasmid DNA is suitable for all molecular-grade applications, as well as in vitro transcription and transfection.
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Resources
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
- Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
- DNA & RNA Selection Guide—Find the right purification products.
- Videos—View our library of DNA & RNA purification and analysis videos.
- Application Notes—Explore our application notes from scientists sharing data for isolation products
For Research Use Only. Not for use in diagnostic procedures.