Bioprocessing Downstream Calculator

This calculator aims to simulate the full chromatography process. For each step, a buffer or solution is suggested as well as a number of CVs (column volumes). Note that you are able to manually input your own number of CVs value or select the suggested value.

For process description, select your modality, target molecule and choose the most appropriate Thermo Fisher recommended chromatography resin. The feedstock concentration unit will automatically update depending on the resin selected.

For chromatography modeling, input the recommended default dynamic binding capacity for the resin. Please specify here if you have predetermined the binding capacity for your specific target molecule.

The safety margin field indicates the level of usage of the resin to mitigate loss of target (e.g., if you plan to load at 80% of the dynamic binding capacity, specify a 20% safety margin).

Enter a value for the column diameter to determine the required volume of packed resin and the corresponding bed height.

It is generally recommended to have a minimum bed height of 2 cm. If the calculated bed height is <2 cm, we recommend decreasing column diameter or using a 1 mL prepacked column.

This buffer calculator uses the Henderson-Hasselbach equation to offer quick references and decisions for the process liquid and buffer solutions critical to the downstream bioprocessing operations.

Input values* from your chromatogram results to calculate symmetry factor, plate counts per meter, reduced plate height, and the height equivalent to a theoretical plate (HETP).