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Rockland
p28ING5 Polyclonal Antibody
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FIGURE: 1 / 2
p28ING5 Antibody (600-101-285) in WB
Western blot analysis is shown using Rocklands Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blo... View More
Product Details
600-101-285
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5).
Conjugate
Form
Liquid
Concentration
1.52 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2. However, 100% homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.
Target Information
ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: inhibitor of growth family, member 5; Inhibitor of growth protein 5; p28ING5
Gene Aliases: ING5; p28ING5
UniProt ID: (Human) Q8WYH8
Entrez Gene ID: (Human) 84289
DNA replication
transcription, DNA-templated
protein acetylation
negative regulation of cell proliferation
chromatin modification
positive regulation of apoptotic process
histone H3 acetylation
positive regulation of transcription, DNA-templated
negative regulation of growth
regulation of signal transduction by p53 class mediator
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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