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Invitrogen
p23 Monoclonal Antibody (JJ6), FITC
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FIGURE: 1 / 4
p23 Antibody (MA5-45196) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human heat shocked cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with p23 monoclonal antibody (Product # MA5-45196) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Product Details
MA5-45196
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
JJ6
Immunogen
Recombinant human full length p23 protein
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
0.5 µg/mL of MA5-45196 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 23kDa.
Target Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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