Invitrogen

nNOS Polyclonal Antibody

FIGURE: 1 / 1

nNOS Antibody (PA1-038) in WB

PloS one 2009 - Figure 10 WT/mdx muscle displays nNOS and dystrobrevin-alpha. Protein extracts from diaphragms of WT (lane 1), 10-30% WT/mdx (lane 2) and mdx (lane 3) mice were subjected to a western blot for dystrophin, nNOS, dystrobrevin-alpha, syntrophin and tubulin. *: dystrobrevin-alpha isoforms enriched in mdx and WT/mdx but not in WT muscle. Experiments were performed with pectoralis, diaphragm and quadriceps mu... View More

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Product Details

PA1-038

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_325775

Product Specific Information

PA1-038 detects recombinant rat brain nitric oxide synthase (nNOS) overexpressed in E. coli. This antibody does not to detect iNOS or eNOS.

PA1-038 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~155 kDa protein representing recombinant rat nNOS protein overexpressed in E. coli.

The PA1-038 immunogen is a synthetic peptide corresponding to residues I(237) Q V D R D L D G K S H K A(250) of rat nNOS.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice.

PloS one

Stillwell E,Vitale J,Zhao Q,Beck A,Schneider J,Khadim F,Elson G,Altaf A,Yehia G,Dong JH,Liu J,Mark W,Bhaumik M,Grange R,Fraidenraich D

PA1-038 was used in western blot to study the rescue of mdx mice by wild type embryonic stem cells

Thu Jul 02 00:00:00 UTC 2009

Altered contractile response due to increased beta3-adrenoceptor stimulation in diabetic cardiomyopathy: the role of nitric oxide synthase 1-derived nitric oxide.

Anesthesiology

Amour J,Loyer X,Le Guen M,Mabrouk N,David JS,Camors E,Carusio N,Vivien B,Andriantsitohaina R,Heymes C,Riou B

PA1-038 was used in western blot to study the role of NOS-1-derived NO in the altered contractile response due to increased beta3-adrenoceptor stimulation in diabetic cardiomyopathy

Sat Sep 01 00:00:00 UTC 2007

Effects of sex differences on constitutive nitric oxide synthase expression and activity in response to pressure overload in rats.

American journal of physiology. Heart and circulatory physiology

Loyer X,Oliviero P,Damy T,Robidel E,Marotte F,Heymes C,Samuel JL

PA1-038 was used in western blot to study gender differences in constitutive NOS expression and activity in pressure overloaded rats

Thu Nov 01 00:00:00 UTC 2007

Reactive nitrogen and oxygen species in airway inflammation.

European journal of pharmacology

Folkerts G,Kloek J,Muijsers RB,Nijkamp FP

PA1-038 was used in immunohistochemistry to review the role of reactive nitrogen and oxygen species in airway inflammation

Fri Oct 19 00:00:00 UTC 2001

Levels of protein kinase C and nitric oxide synthase activity in rats exposed to sub chronic low level lead.

Molecular and cellular biochemistry

Ramesh GT,Jadhav AL

PA1-038 was used in western blot to study levels of protein kinase C and NOS activity in brain regions of rats exposed to sub chronic low level lead

Sun Jul 01 00:00:00 UTC 2001

Neuronal nitric-oxide synthase-mu, an alternatively spliced isoform expressed in differentiated skeletal muscle.

The Journal of biological chemistry

Silvagno F,Xia H,Bredt DS

PA1-038 was used in western blot to investigate the distribution of nitric-oxide synthase mu in differentiated skeletal muscle

Fri May 10 00:00:00 UTC 1996

Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.

Nature

Bredt DS,Hwang PM,Glatt CE,Lowenstein C,Reed RR,Snyder SH

PA1-038 was used in western blot to investigate the characteristics of nitric oxide synthase in vivo

Thu Jun 27 00:00:00 UTC 1991

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