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Invitrogen
mDIC Polyclonal Antibody
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FIGURE: 1 / 6
mDIC Antibody (PA3-840) in ICC/IF
Immunofluorescent analysis of MDC1 (green) showing staining in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MDC1 polyclonal antibody (Product # PA3-840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLigh... View More
Product Details
PA3-840
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal residues L(276) R K H F G I K V P T T(287) of mDIC.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_560041
Product Specific Information
PA3-840 detects mitochondrial dicarboxylate carrier protein(mDIC) in human and mouse cells.
PA3-840 has been successfully used in Western blotting and ICC/IF procedures. By Western blot it detects a 28 kDa protein representing mDIC.
The PA3-840 immunogen is a synthetic peptide corresponding to the C-terminal residues L(276) R K H F G I K V P T T(287) of mDIC.
Target Information
The mitochondrial dicarboxylate carrier protein (mDIC) belongs to superfamily of mitochondrial transporters. It has been demonstrated that the expression of mDIC leads to hyperpolarization of the mitochondria, as well as succinate uptake by the mitochondria. It is highly expressed in adipose tissue, where its expression is downregulated by insulin and upregulated by free fatty acids. mDIC is also thought to play a role in glyceroneogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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