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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • beta Actin Antibodies
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          Invitrogen

          beta Actin Polyclonal Antibody

          12 Published Figures
          31 References
          View all (96) beta Actin antibodies

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          Datasheet
          Protocols
          Datasheet
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          Cite beta Actin Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (12)
          beta Actin Antibody in Immunocytochemistry (ICC/IF)
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          beta Actin Antibody in Immunocytochemistry (ICC/IF)
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          FIGURE: 1 / 16

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          beta Actin Antibody (PA5-16914) in ICC/IF

          Immunofluorescence analysis of beta Actin was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Actin Rabbit polyclonal antibody (Product # PA5-16914) at 2 µg/mL in 0.1% BSA and incubated for 3 hou... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          beta Actin Antibody in Immunocytochemistry (ICC/IF)
          beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)
          beta Actin Antibody in Western Blot (WB)

          Product Details

          PA5-16914

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 25 publications 25 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          5 µg/mL
          -
          Product Specifications

          Published species

          Dog, Fruit fly, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to N-terminus of human beta-actin
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10976894

          Product Specific Information

          PA5-16914 targets Actin Beta in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Chicken, Drosophila, Feline, Guinea Pig, Human, Leech, mouse, Ovine, Porcine, Rabbit, and Rat samples.

          The PA5-16914 immunogen is synthetic peptide corresponding to N-terminus of human beta-actin.

          Target Information

          Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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