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Invitrogen
alpha-1 Antitrypsin Polyclonal Antibody
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FIGURE: 1 / 4
alpha-1 Antitrypsin Antibody (PA5-118256) in WB
Western Blot using alpha-1 Antitrypsin Polyclonal Antibody (Product # PA5-118256) at 1:500 dilution. Lane A: Rat Liver tissue lysate, Lane B: Rat Spleen tissue lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 46 kDa. Observed band size: 54 kDa.
Product Details
PA5-118256
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Rat SerpinA1 Protein
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2902855
Target Information
Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a-1 AT; a1-antitrypsin; alpha 1 antitrypsin; Alpha-1-antiproteinase; Alpha-1-antitrypsin; alpha-1-antitrypsin (protease inhibitor); alpha-1-protease inhibitor; Alpha-1-proteinase inhibitor; Digestive Zymogen; MGC23330; MGC9222; Protease Serine 1; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1; Serine Protease 1; serine protease inhibitor alpha 1; Serpin A1; TRP1; TRY1
Gene Aliases: AAT; Pi; Serpina1; Spi1
UniProt ID: (Rat) P17475
Entrez Gene ID: (Rat) 24648
response to hypoxia
acute-phase response
inflammatory response
response to inorganic substance
response to lead ion
response to organic cyclic compound
response to estradiol
response to lipopolysaccharide
response to methanol
response to triglyceride
response to cytokine
response to chromate
negative regulation of serine-type endopeptidase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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