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Invitrogen
ZNF451 Polyclonal Antibody
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FIGURE: 1 / 1
ZNF451 Antibody (PA5-116621) in WB
Western blot analysis of ZNF451 in extracts of various cell lines. Samples were incubated with ZNF451 Polyclonal antibody (Product # PA5-116621) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-116621
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 600-900 of human ZNF451 (NP_0010267941)
Conjugate
Form
Liquid
Concentration
1.52 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901252
Product Specific Information
Cellular location: Nucleus
Immunogen sequence: SSPRGKWQCR ICEDMFDSQE YVKQHCMSLA SHKFHRYSCA HCRKPFHKIE TLYRHCQDEH DNEIKIKYFC GLCDLIFNVE EAFLSHYEEH HSIDYVFVSE KTETSIKTED DFPVIETSNQ LTCGCRESYI CKVNRKEDYS RCLQIMLDKG KLWFRCSLCS ATAQNLTDMN THIHQVHKEK SDEEEQQYVI KCGTCTKAFH DPESAQQHFH RKHCFLQKPS VAHFGSEKSN LYKFTASASH TERKLKQAIN YSKSLDMEKG VENDLSYQNI EEEIVELPDL DYLRTMTHIV FVDFDNWSNF F
Target Information
E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins (PubMed:26524493, PubMed:26524494). Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11' (PubMed:26524493). Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9' (PubMed:24324267). Plays a role in regulating the transcription of AR targets (PubMed:18656483). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coactivator for steroid receptors; E3 SUMO-protein ligase ZNF451; E3 SUMO-protein transferase ZNF451; Zinc finger protein 451
Gene Aliases: 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; dJ417I1.1; KIAA0576; Kiaa0576-hp; KIAA1702; mKIAA1702; Zfp451; ZNF451
UniProt ID: (Human) Q9Y4E5, (Mouse) Q8C0P7
Entrez Gene ID: (Human) 26036, (Rat) 316312, (Mouse) 98403
transcription, DNA-templated
protein sumoylation
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of transcription initiation from RNA polymerase II promoter
negative regulation of histone H3-K9 acetylation
regulation of transcription, DNA-templated
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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