Invitrogen

YAP1 Polyclonal Antibody

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YAP1 Antibody (PA5-13504) in WB

Western blot analysis using a YAP1 polyclonal antibody (Product # PA5-13504) in NCI-H460 cell lysates (35 µg per lane).

Product Image Gallery

YAP1 Antibody — Figure 4 YAP siRNA knockdown allows re-sensitization to EGFR inhibitors A. H1975/OR sub-line shows reduced cell viability when re-introduced to osimertinib after YAP silencing, especially evident at doses around 10 muM. B. We observed HCC827/ER respond to erlotinib after siRNA silencing of YAP from 1 muM drug, with gradually pronounced difference from non-silenced cells up 10 muM. C. HCC827/GR sub-line showed decrease in cell viability after the introduction of drug and silencing of YAP. All siRNA knockdown wells were normalised to their negative siRNA drug counterpart and standard error was calculated based upon two repeats. D. siRNA knockdown was confirmed using Western Blot with GAPDH as a loading control. ** p = <0.05. {KD}

Product Details

PA5-13504

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 420-446 amino acids from the C-terminal region of human YAP1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Lot-specific

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2219136

Target Information

YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NSCLC depend upon YAP expression and nuclear localization after acquiring resistance to EGFR inhibitors.

Genes & cancer

McGowan M,Kleinberg L,Halvorsen AR,Helland Å,Brustugun OT

Published figure using YAP1 polyclonal antibody (Product # PA5-13504) in Western Blot

Wed Mar 01 00:00:00 UTC 2017

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