Invitrogen

XBP1 Polyclonal Antibody

XBP1 Antibody in Immunocytochemistry (ICC/IF)

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XBP1 Antibody (PA5-27650) in ICC/IF

Immunofluorescence analysis of XBP1 was performed using 70% confluent log phase MCF7 cells treated with thapsigargin (2uM, 6hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with XBP1 Polyclonal Antibody (Product # PA5-27650) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa F... View More

XBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

XBP1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

XBP1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

XBP1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of XBP1 using with XBP1 Polyclonal Antibody (Product # PA5-27650) shows an increase upon thapsigargin treatment. {TM}

Product Details

PA5-27650

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 4 publications 4 publications

Product Specifications

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 90 and 261 of Human XBP1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.93 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2545126

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, HepG2 (3 µM Thapsigargin treatment for 24 hr).

Predicted reactivity: Pig (84%), Rhesus Monkey (98%), Bovine (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Molecular Mechanisms of the Cytotoxic Effect of Recombinant Selenoprotein SELENOM on Human Glioblastoma Cells.

International journal of molecular sciences

Rogachev VV,Goltyaev MV,Varlamova EG,Turovsky EA

PA5-27650 was used in Western Blot to demonstrate a dose-dependent depletion of the Ca2+ pool under the action of SELENOM, which proves the important role of this protein in the regulation of calcium homeostasis in the cell.

Thu Mar 30 00:00:00 UTC 2023

Aging compromises human islet beta cell function and identity by decreasing transcription factor activity and inducing ER stress.

Science advances

Shrestha S,Erikson G,Lyon J,Spigelman AF,Bautista A,Manning Fox JE,Dos Santos C,Shokhirev M,Cartailler JP,Hetzer MW,MacDonald PE,Arrojo E Drigo R

PA5-27650 was used in Immunohistochemistry to propose that a chronic state of endoplasmic reticulum (ER) stress undermines old beta cell structure function to increase the risk of beta cell failure and type 2 diabetes onset as humans age.

Fri Oct 07 00:00:00 UTC 2022

The Role of Selenoproteins SELENOM and SELENOT in the Regulation of Apoptosis, ER Stress, and Calcium Homeostasis in the A-172 Human Glioblastoma Cell Line.

Biology

Varlamova EG,Goltyaev MV,Turovsky EA

PA5-27650 was used in Western Blotting to investigate the mechanisms of fine regulation of the processes of apoptosis, cell proliferation, and ER stress by two ER resident proteins, SELENOM and SELENOT.

Wed May 25 00:00:00 UTC 2022

Porcine oviductal extracellular vesicles facilitate early embryonic development via relief of endoplasmic reticulum stress.

Cell biology international

Fu B,Ma H,Zhang DJ,Wang L,Li ZQ,Guo ZH,Liu ZG,Wu SH,Meng XR,Wang F,Chen WG,Liu D

PA5-27650 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that the maternal-embryo communication mediated by oviductal EVs benefits early embryonic development.

Tue Feb 01 00:00:00 UTC 2022

Zinc supplementation alleviates endoplasmic reticulum stress during porcine oocyte in vitro maturation by upregulating zinc transporters.

Journal of cellular physiology

Ridlo MR,Kim GA,Taweechaipaisankul A,Kim EH,Lee BC

Published figure using XBP1 polyclonal antibody (Product # PA5-27650) in Immunocytochemistry

Thu Apr 01 00:00:00 UTC 2021

Adiponectin Improves In Vitro Development of Cloned Porcine Embryos by Reducing Endoplasmic Reticulum Stress and Apoptosis.

Animals : an open access journal from MDPI

Ridlo MR,Kim EH,Taweechaipaisankul A,Lee BC,Kim GA

PA5-27650 was used in Immunocytochemistry and Immunohistochemistry to suggest that 15 µg/mL adiponectin enhanced the in vitro developmental capacity of early-stage SCNT porcine embryos by reducing ER stress and apoptosis.

Wed Feb 10 00:00:00 UTC 2021

Adiponectin Improves In Vitro Development of Cloned Porcine Embryos by Reducing Endoplasmic Reticulum Stress and Apoptosis.

Animals : an open access journal from MDPI

Ridlo MR,Kim EH,Taweechaipaisankul A,Lee BC,Kim GA

Wed Feb 10 00:00:00 UTC 2021

MicroRNA-210 Regulates Endoplasmic Reticulum Stress and Apoptosis in Porcine Embryos.

Animals : an open access journal from MDPI

Ridlo MR,Kim EH,Kim GA

PA5-27650 was used in Immunocytochemistry and Immunohistochemistry to support the enhancement of in vitro embryo production.

Mon Jan 18 00:00:00 UTC 2021

MicroRNA-210 Regulates Endoplasmic Reticulum Stress and Apoptosis in Porcine Embryos.

Animals : an open access journal from MDPI

Ridlo MR,Kim EH,Kim GA

PA5-27650 was used in Immunocytochemistry and Immunohistochemistry to support the enhancement of in vitro embryo production.

Mon Jan 18 00:00:00 UTC 2021

Effect of Denervation on XBP1 in Skeletal Muscle and the Neuromuscular Junction.

International journal of molecular sciences

Walter LA,Blake LP,Gallot YS,Arends CJ,Sozio RS,Onifer SM,Bohnert KR

PA5-27650 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that all three arms of the unfolded protein response (UPR) are activated in denervated skeletal muscle.

Fri Dec 24 00:00:00 UTC 2021

EGFR targeting enhances the efficiency of chemotherapy through inhibiting IRE1α-XBP1s pathway in colorectal cancer cells.

Journal of Cancer

Huo M,Zhao Y,Liu X,Gao Y,Zhang D,Chang M,Liu M,Xu N,Zhu H

PA5-27650 was used in Western Blotting to detect the activation of IRE1α-XBP1s signaling in patient cancer tissues and colorectal cancer cell lines.

Mon Sep 28 00:00:00 UTC 2020

CCDC170 affects breast cancer apoptosis through IRE1 pathway.

Aging