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Invitrogen
VPS4B Polyclonal Antibody
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FIGURE: 1 / 1
VPS4B Antibody (PA5-90678) in WB
Western blot analysis of VPS4B in extracts of various cell lines. Samples were incubated with VPS4B Polyclonal antibody (Product # PA5-90678) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-90678
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human VPS4B (NP_0048602)
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806171
Product Specific Information
Immunogen sequence: MSSTSPNLQK AIDLASKAAQ EDKAGNYEEA LQLYQHAVQY FLHVVKYEAQ GDKAKQSIRA KCTEYLDRAE KLKEYLKNKE KKAQKPVKEG QPSPADEKGN DSDGEGESDD PEKKKLQNQL QGAIVIERPN; Positive Samples: Mouse kidney, Mouse heart; Cellular Location: Late endosome membrane, Peripheral membrane protein, Prevacuolar compartment membrane
Target Information
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein SKD1; Suppressor of K(+) transport growth defect 1; suppressor of K+ transport defect 1; Vacuolar protein sorting-associated protein 4B
Gene Aliases: 8030489C12Rik; Skd1; Vps4b
UniProt ID: (Mouse) P46467
Entrez Gene ID: (Mouse) 20479
cell separation after cytokinesis
transport
potassium ion transport
nucleus organization
endosome organization
vacuole organization
cell cycle
mitotic metaphase plate congression
regulation of centrosome duplication
positive regulation of G2/M transition of mitotic cell cycle
protein transport
endosomal transport
viral release from host cell
cholesterol transport
endosome to lysosome transport via multivesicular body sorting pathway
response to lipid
viral budding via host ESCRT complex
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
positive regulation of viral process
regulation of viral process
protein depolymerization
cell division
negative regulation of cell death
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
regulation of mitotic spindle assembly
positive regulation of viral release from host cell
negative regulation of exosomal secretion
positive regulation of exosomal secretion
positive regulation of centriole elongation
positive regulation of viral life cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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