Invitrogen

VPS34 Polyclonal Antibody

FIGURE: 1 / 2

VPS34 Antibody (PA5-28684) in WB

Western blot analysis of PI 3 Kinase Class 3 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PI 3 Kinase Class 3 polyclonal antibody (Product # PA5-28684) at a dilution of 1:1000.

Product Image Gallery

VPS34 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with PIK3C3 shRNA and decrease in signal intensity was observed in western blot application using PI 3 Kinase Class 3 polyclonal antibody (Product # PA5-28684). {KD}

Product Details

PA5-28684

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 492 and 887 of Human PI 3 Kinase Class 3

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2546160

Product Specific Information

Recommended positive controls: A431, 293T.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (91%), Xenopus laevis (95%), Dog (100%), Pig (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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