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Invitrogen
VAMP2 Polyclonal Antibody
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FIGURE: 1 / 4
VAMP2 Antibody (OSS00035W) in ICC/IF
Immunofluorescence analysis of Vesicle-associated membrane protein 2 was performed using 70% confluent log phase U-87 MG and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VAMP2 Polyclonal Antibody (Product # OSS00035W), at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Al... View More
Product Details
OSS00035W
Applications
Tested Dilution
Publications
Product Specifications
Published species
Dog
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 10-60 of human VAMP2 conjugated to blue carrier protein was used as the antigen.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Wet ice
RRID
AB_1960853
Product Specific Information
Glycerol (1:1) may be added for additional stability.
Reconstitute in 100 µL of sterile water.
The VAMP2 peptide shares 93% shared identity with VAMP1. The peptide is homologous in many other species including rat, mouse and xenopus.
Target Information
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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