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Invitrogen
UBE2J1 Polyclonal Antibody
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FIGURE: 1 / 2
UBE2J1 Antibody (PA5-19675) in ICC/IF
Immunofluorescent staining of HeLa cells using Product # PA5-19675, anti-UBC6e antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 5 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain ... View More
Product Details
PA5-19675
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human UBC6e.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10987118
Product Specific Information
PA5-19675 targets UBC6e in IF and WB applications and shows reactivity with Human samples.
The PA5-19675 immunogen is synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human UBC6e.
PA5-19675 detects UBC6e which has a predicted molecular weight of approximately 37 kDa.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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