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Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is thyroid or lung carcinoma.
TTF1 is a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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