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Invitrogen
TRIM23 Polyclonal Antibody
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FIGURE: 1 / 5
TRIM23 Antibody (PA5-34624) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TRIM23 using TRIM23 Polyclonal Antibody (Product # PA5-34624). Samples used were HCT116 cells mock (left) and cells treated with 30 µM Cisplatin for 24 hrs (right) fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 Polyclonal Antibody (Product # PA5-34624) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.
Product Details
PA5-34624
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TRIM23. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2551976
Product Specific Information
Recommended positive controls: HeLaS3.
Predicted reactivity: Rhesus Monkey (100%).
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ADP-ribosylation factor domain protein 1, 64kD; ADP-ribosylation factor domain protein 1, 64kDa; ADP-ribosylation factor domain-containing protein 1; ARF domain protein 1; E3 ubiquitin-protein ligase TRIM23; GTP-binding protein ARD-1; RING finger protein 46; RING-type E3 ubiquitin transferase TRIM23; tripartite motif protein 23; tripartite motif protein TRIM23; tripartite motif-containing 23; Tripartite motif-containing protein 23
Gene Aliases: 6330516O20Rik; AI450195; ARD1; ARFD1; RNF46; TRIM23
UniProt ID: (Human) P36406, (Mouse) Q8BGX0
Entrez Gene ID: (Human) 373, (Mouse) 81003
GTPase activity
ubiquitin-protein transferase activity
protein binding
GTP binding
enzyme activator activity
zinc ion binding
ligase activity
GDP binding
identical protein binding
nucleotide binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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