Invitrogen

STAT1 Polyclonal Antibody

STAT1 Antibody in Immunohistochemistry (IHC)

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STAT1 Antibody (PA5-34504) in IHC

Immunofluorescent analysis of human small intestine tissue using a STAT1 polyclonal antibody (Product # PA5-34504) at a 20 µg/mL dilution.

Product Image Gallery

STAT1 Antibody — Fig. 4 AFB 1 inhibits STAT1 protein synthesis. HepG2 cells were cultured at density of 5 x 10 4 cells per well of the 6-well plate until they reached 80% confluence. The cells were stimulated and treated as described above. After blotting, the membranes were immunoblotted with antibody against the STAT1 epitope of the STAT1 protein as primary antibody followed by horseradish peroxidase conjugated goat anti-rabbit antibody as secondary antibody. The membranes were also probed for GAPDH which was used as endogenous control. The dilutions of the primary antibodies used against STAT1 and GAPDH proteins were as follows: STAT1 (Thermo Scientific, cat no PA5-34504; 1:1000), GAPDH (Thermo Scientific, cat no QE212271; 1:2500). The secondary antibody used was polyclonal goat anti-Rabbit conjugated to HRP (Thermo Scientific, cat no PI31460, 1: 5000). The blotted membranes were developed using enhanced chemiluminescence reagents and the protein bands were detected and captured with C-DIGIT blot scanner imaging device. ( a ) The relative STAT1 protein intensity was quantified using Image J software package. ( b ) Western blot analysis of STAT1 protein. ( c ) Western blot analysis of GAPDH protein as loading control {TM}

Product Details

PA5-34504

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

20 µg/mL

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid peptide near the carboxy terminus of human STAT1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Wet ice

AB_2551856

Product Specific Information

A suggested positive control is human small intestine tissue lysate.

PA5-34504 can be used with blocking peptide PEP-1547.

Target Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aflatoxin B₁ inhibits the type 1 interferon response pathway via STAT1 suggesting another mechanism of hepatocellular carcinoma.

Infectious agents and cancer

Narkwa PW,Blackbourn DJ,Mutocheluh M

Published figure using STAT1 polyclonal antibody (Product # PA5-34504) in Western Blot

Sun Nov 12 00:00:00 UTC 2023

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