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Invitrogen
SP5 Polyclonal Antibody
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FIGURE: 1 / 3
SP5 Antibody (PA5-103505) in ICC/IF
Immunocytochemistry analysis of SP5 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SP5 polyclonal antibody (Product # PA5-103505) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103505
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SP5(Accession Q6BEB4), corresponding to amino acid residues T260-T310.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852841
Product Specific Information
Antibody detects endogenous levels of total SP5.
Target Information
SP5 binds to GC boxes promoters elements. SP5 probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Transcription factor Sp5
Gene Aliases: SP5
UniProt ID: (Human) Q6BEB4, (Mouse) Q9JHX2
Entrez Gene ID: (Human) 389058, (Mouse) 64406
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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