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Invitrogen
SP1 Polyclonal Antibody
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FIGURE: 1 / 9
SP1 Antibody (PA1-25578) in ICC/IF
Immunofluorescent analysis of SP1 (green) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a SP1 polyclonal antibody (Product # PA1-25578) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) ... View More
Product Details
PA1-25578
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal within the region between residues 750-785 of human SP1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
PA1-25578 detects SP1 from human samples. PA1-25578 is expected to cross react with mouse (96% conserved), rat, Xenopus laevis (86% conserved), bovine (98% conserved), zebrafish (100% conserved), chicken (88% conserved), and porcine (95% conserved) due to sequence homology.
PA1-25578 has been successfully used in Western blot, FACS, IHC and immunoprecipitation procedures. The predicted molecular weight of PA1-25578 is 90 kDa.
The PA1-25578 immunogen is a synthetic peptide corresponding to the C-terminal within the region between residues 750-785 of human SP1.
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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