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Invitrogen
SIRT3 Polyclonal Antibody
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FIGURE: 1 / 2
SIRT3 Antibody (PA5-19732) in ICC/IF
Immunofluorescent staining of HeLa cells using Product # PA5-19732, anti-SIRT3 antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with TBS-T (20mins), BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red)... View More
Product Details
PA5-19732
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SIRT3.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10981824
Product Specific Information
PA5-19732 targets SIRT3 in IF and WB applications and shows reactivity with Human samples.
The PA5-19732 immunogen is synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SIRT3.
PA5-19732 detects SIRT3 which has a predicted molecular weight of approximately 50 kDa.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
Target Information
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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