Invitrogen

SEC61A1 Polyclonal Antibody

FIGURE: 1 / 3

SEC61A1 Antibody (PA3-014) in WB

Western blot of sec61 alpha in pig microsomes using Product # PA3-014.

SEC61A1 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

PA3-014

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(13) V I L P E I Q K P E R K I Q F K E K(31) of canine SEC61 alpha.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2186390

Product Specific Information

PA3-014 detects human, canine and porcine, rat and mouse sec61 alpha from microsomes and shows no cross-reactivity with other sec61 isoforms.

PA3-014 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~38 kDa protein representing sec61 alpha from pig microsomes.

The PA3-014 immunogen is a synthetic peptide corresponding to residues C(13) V I L P E I Q K P E R K I Q F K E K(31) of canine sec61 alpha. This sequence is completely conserved between human, mouse, rat, and canine.

Target Information

The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Molecular mechanism of ER stress-induced pre-emptive quality control involving association of the translocon, Derlin-1, and HRD1.

Scientific reports

Kadowaki H,Satrimafitrah P,Takami Y,Nishitoh H

PA3-014 was used in Western Blotting to study the mechanism by which endoplasmic reticulum stress-induced pre-emptive quality control substrates are captured and degraded.

Wed May 09 00:00:00 UTC 2018

Detection of Temperature Difference in Neuronal Cells.

Scientific reports

Tanimoto R,Hiraiwa T,Nakai Y,Shindo Y,Oka K,Hiroi N,Funahashi A

Published figure using SEC61A1 polyclonal antibody (Product # PA3-014) in Immunofluorescence

Tue Mar 01 00:00:00 UTC 2016

Transport of estradiol-17β-glucuronide, estrone-3-sulfate and taurocholate across the endoplasmic reticulum membrane: evidence for different transport systems.

Biochemical pharmacology

Wlcek K,Hofstetter L,Stieger B

PA3-014 was used in western blot to study the involvement of different systems in the ER transport of estradiol-17beta-glucuronide, estrone-3-sulfate and taurocholate

Sat Mar 01 00:00:00 UTC 2014

Translocon pores in the endoplasmic reticulum are permeable to small anions.

American journal of physiology. Cell physiology

Lizák B,Czegle I,Csala M,Benedetti A,Mandl J,Bánhegyi G

PA3-014 was used in western blot to study the permeability of small ions through endoplasmic reticulum

Fri Sep 01 00:00:00 UTC 2006

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more