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Invitrogen
SCN10A Polyclonal Antibody
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FIGURE: 1 / 2
SCN10A Antibody (PA5-111804) in IHC
Immunohistochemistry analysis of SCN10A in rat DRG tissue sections using SCN10A Antibody (Product # PA5-111804). A) NaV1.8 staining (green) appears in the cell bodies of the DRG neurons. B) Nuclear staining using DAPI as the counter stain (blue). C) Merged image of A and B.
Product Details
PA5-111804
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Guinea pig
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Intracellular, C-terminus)
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2857213
Product Specific Information
Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Peptide sequence is: (C)EDEVAAK EGNSPGPQ
Homology: Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.
Target Information
Scn10a is a tetrodotoxin-resistant voltage-gated sodium channel alpha subunit. Epithelial sodium channels are amiloride-sensitive members of the Degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Scn10a mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Scn10a forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Scn10a also plays a role in neuropathic pain mechanisms. Structurally, members of Deg/ENaC superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddels syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1). Diseases associated with SCN10A include Episodic Pain Syndrome, Familial, 2 and Sodium Channelopathy-Related Small Fiber Neuropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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