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SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Polyclonal Antibody

SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody in Western Blot (WB)

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SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody (PA5-81795) in WB

Western Blot analysis using a SARS Coronavirus Spike Protein (subunit 1) antibody (Product # PA5-81795). Proteins were loaded on a reducing SDS-PAGE. Lane A: SARS-CoV Spike S1, 5 ng; Lane B: SARS-CoV2 Spike S1, 30 ng; Lane C: SARS-CoV2 Spike RBD, 30 ng. Separated proteins were transferred to a membrane and probed with PA5-81795 at 1:2000 dilution. The membrane was then probed with goat anti-rabbit IgG (H+L)... View More

SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody in Immunocytochemistry (ICC/IF)

SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-81795

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

1:5,000-1:10,000

Neutralization (Neu)

View 1 publication 1 publication

Immunoelectrophoresis (IE)

View 1 publication 1 publication

Product Specifications

Published species

Human, Plant, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human SARS Coronavirus Spike S1 Subunit Protein (Met1-Arg667)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Wet ice

AB_2788969

Product Specific Information

PA5-81795 has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Spike S1 and SARS-CoV-2 (2019-nCoV) Spike RBD.

PA5-81795 was prepared using the sequence encoding the S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667). This was expressed with a C-terminal polyhistidine tag.

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human SARS Coronavirus Spike S1 Subunit Protein.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Infectious SARS-CoV-2 Particles from Rectal Swab Samples from COVID-19 Patients in Brazil.

Viruses

Ribeiro IP,Nascimento LGD,Tort LFL,Pereira EC,Menezes LSR,Malta FC,Oliveira BCEPD,Rodrigues JP,Manso PPA,Pelajo M,Bonaldo MC,Silva PCR,Siqueira MM,Brasil P,Fumian TM

PA5-81795 was used in Immunocytochemistry to demonstrate the replication capacity of SARS-CoV-2 in the GI tract, and infectious viruses in one RS sample.

Thu May 11 00:00:00 UTC 2023

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in Chronic Obstructive Pulmonary Disease.

Microbiology spectrum

Osan J,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

PA5-81795 was used in Immunohistochemistry to determine whether SARS-CoV-2's cellular tropism plays a critical role in severe pathophysiology in the lung.

Wed Aug 31 00:00:00 UTC 2022

Concentration of SARS-CoV-2-Infected Cell Culture Supernatants for Detection of Virus-like Particles by Scanning Electron Microscopy.

Viruses

Le Bideau M,Robresco L,Baudoin JP,La Scola B

PA5-81795 was used in ELISA, Western Blot, Immunohistochemistry to find that centrifugation of 25XConcanavalinA-coated microscopy glass slides in shell vials provided an improved method for concentrating SARS-CoV-2-virus-infected cell supernatants for virus-like particle detection by SEM.

Fri Oct 28 00:00:00 UTC 2022

Scalable agroinfiltration-based production of SARS-CoV-2 antigens for use in diagnostic assays and subunit vaccines.

PloS one

Demone J,Maltseva M,Nourimand M,Nasr-Sharif M,Galipeau Y,Alarcon EI,Langlois MA,MacLean AM

PA5-81795 was used in Western Blotting to demonstrate that recombinant RBD produced via agroinfiltration exhibits suitable biochemical and antigenic features for use in serological and neutralization assays, and in subunit vaccine platforms.

Fri Dec 16 00:00:00 UTC 2022

Spike Protein Subunits of SARS-CoV-2 Alter Mitochondrial Metabolism in Human Pulmonary Microvascular Endothelial Cells: Involvement of Factor Xa.

Disease markers

Zekri-Nechar K,Zamorano-León JJ,Reche C,Giner M,López-de-Andrés A,Jiménez-García R,López-Farré AJ,Martínez-Martínez CH

PA5-81795 was used in Western Blotting to analyze if in human pulmonary microvascular endothelial cells (HPMEC), the SARS-CoV-2 (COVID-19) spike protein subunits, S1 and S2 (S1+S2), could alter mitochondrial metabolism and what is the role of FXA.

Fri Dec 09 00:00:00 UTC 2022

Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies.

PLoS pathogens

Alenquer M,Ferreira F,Lousa D,Valério M,Medina-Lopes M,Bergman ML,Gonçalves J,Demengeot J,Leite RB,Lilue J,Ning Z,Penha-Gonçalves C,Soares H,Soares CM,Amorim MJ

PA5-81795 was used in Neutralization to engineer SARS-CoV-2 spike-pseudotyped viruses and analyse individual and combined mutations that convergently appeared in different lineages, over time and across several geographic locations, to determine their synergetic effects on neutralizing-antibody responses.

Sun Aug 01 00:00:00 UTC 2021

Microscopic Observation of SARS-Like Particles in RT-qPCR SARS-CoV-2 Positive Sewage Samples.

Pathogens (Basel, Switzerland)

Brahim Belhaouari D,Wurtz N,Grimaldier C,Lacoste A,Pires de Souza GA,Penant G,Hannat S,Baudoin JP,La Scola B

PA5-81795 was used in Immunocytochemistry and Immunohistochemistry to investigate the possible presence of SARS-CoV-2 particles in Marseille wastewater using correlative light-electron microscopy and check the viability of the virus.

Sat Apr 24 00:00:00 UTC 2021

Microscopic Observation of SARS-Like Particles in RT-qPCR SARS-CoV-2 Positive Sewage Samples.

Pathogens (Basel, Switzerland)

Brahim Belhaouari D,Wurtz N,Grimaldier C,Lacoste A,Pires de Souza GA,Penant G,Hannat S,Baudoin JP,La Scola B

Sat Apr 24 00:00:00 UTC 2021

Microscopic Observation of SARS-Like Particles in RT-qPCR SARS-CoV-2 Positive Sewage Samples.

Pathogens (Basel, Switzerland)

Brahim Belhaouari D,Wurtz N,Grimaldier C,Lacoste A,Pires de Souza GA,Penant G,Hannat S,Baudoin JP,La Scola B

Sat Apr 24 00:00:00 UTC 2021

Rapid Detection of SARS-CoV-2 Antigens Using High-Purity Semiconducting Single-Walled Carbon Nanotube-Based Field-Effect Transistors.

ACS applied materials & interfaces

Shao W,Shurin MR,Wheeler SE,He X,Star A

PA5-81795 was used in Immunoelectrophoresis to assess the presence of SARS-CoV-2 antigens in clinical nasopharyngeal samples.

Wed Mar 03 00:00:00 UTC 2021

Arginine methylation of SARS-Cov-2 nucleocapsid protein regulates RNA binding, its ability to suppress stress granule formation, and viral replication.

The Journal of biological chemistry

Cai T,Yu Z,Wang Z,Liang C,Richard S

PA5-81795 was used in Western Blotting to propose that type 1 PRMT inhibitors can suppress arginine methylation of SARS-CoV-2 to target the later stages of the viral life cycle.

Thu Jul 01 00:00:00 UTC 2021

A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19.

NPJ vaccines

Malherbe DC,Kurup D,Wirblich C,Ronk AJ,Mire C,Kuzmina N,Shaik N,Periasamy S,Hyde MA,Williams JM,Shi PY,Schnell MJ,Bukreyev A

PA5-81795 was used in Western Blot to design and produce ConVac, a replication-competent vesicular stomatitis virus (VSV) vaccine vector that expresses the S1 subunit of SARS-CoV-2 spike protein.

Thu Jul 22 00:00:00 UTC 2021

Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System.

Biological procedures online

Shang Y,Chen F,Li S,Song L,Gao Y,Yu X,Zheng J

PA5-81795 was used in Western Blot to provide a new in vitro system for investigating the interaction between SARS-CoV-2 and host cells and purifying ACE2-expressing cells.

Thu Aug 26 00:00:00 UTC 2021

Presence of SARS-CoV-2 in a Cornea Transplant.

Pathogens (Basel, Switzerland)

Otmani Idrissi M,Baudoin JP,Chateau AL,Aherfi S,Bedotto-Buffet M,Latil A,Lepidi H,Chiaroni J,Picard C,Mege JL,La Scola B,Mezouar S

PA5-81795 was used in Immunohistochemistry to suggest important implications for the use of corneal tissue in graft indications and open the debate on SARS-CoV-2 transmissibility.

Sat Jul 24 00:00:00 UTC 2021

Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation.

Vaccines

Farnós O,Venereo-Sánchez A,Xu X,Chan C,Dash S,Chaabane H,Sauvageau J,Brahimi F,Saragovi U,Leclerc D,Kamen AA

Published figure using SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) polyclonal antibody (Product # PA5-81795) in Western Blot

Wed Nov 04 00:00:00 UTC 2020

TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes.

Science immunology

Zang R,Gomez Castro MF,McCune BT,Zeng Q,Rothlauf PW,Sonnek NM,Liu Z,Brulois KF,Wang X,Greenberg HB,Diamond MS,Ciorba MA,Whelan SPJ,Ding S

PA5-81795 was used in Western blotting to highlight the intestine as a potential site of SARS-CoV-2 replication, which may contribute to local and systemic illness and overall disease progression.

Wed May 13 00:00:00 UTC 2020

TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes.

Science immunology

Zang R,Gomez Castro MF,McCune BT,Zeng Q,Rothlauf PW,Sonnek NM,Liu Z,Brulois KF,Wang X,Greenberg HB,Diamond MS,Ciorba MA,Whelan SPJ,Ding S

PA5-81795 was used in Western blotting to highlight the intestine as a potential site of SARS-CoV-2 replication, which may contribute to local and systemic illness and overall disease progression.

Wed May 13 00:00:00 UTC 2020

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.

bioRxiv : the preprint server for biology

Osan JK,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

PA5-81795 was used in Immunohistochemistry (PFA fixed) to study SARS-CoV-2 infection in healthy and COPD lung cells.

Thu Nov 12 00:00:00 UTC 2020

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.

bioRxiv : the preprint server for biology

Osan JK,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

PA5-81795 was used in Immunohistochemistry (PFA fixed) to study SARS-CoV-2 infection in healthy and COPD lung cells.

Thu Nov 12 00:00:00 UTC 2020

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SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Polyclonal Antibody

SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody (PA5-81795) in WB

Product Details

PA5-81795

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Neutralization (Neu)

Immunoelectrophoresis (IE)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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