Invitrogen

S100 Polyclonal Antibody

S100 Antibody in Immunocytochemistry (ICC/IF)

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S100 Antibody (PA5-16257) in ICC/IF

Immunofluorescence analysis of S100 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with S100 Rabbit Polyclonal Antibody (Product # PA5-16257) at 1:250 in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More

S100 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-16257

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2500

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Product Specifications

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

S100 protein purified from cow brain

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

4°C

Shipping conditions

Wet ice

AB_10984701

Product Specific Information

PA5-16257 targets S100 Protein in ICC and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, Marsupial, mouse, and Rat samples.

The PA5-16257 immunogen is s100 protein purified from cow brain.

Target Information

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Papillary-cystic pattern is characteristic in mammary analogue secretory carcinomas but is rarely observed in acinic cell carcinomas of the salivary gland.

Virchows Archiv : an international journal of pathology

Hsieh MS,Chou YH,Yeh SJ,Chang YL

PA5-16257 was used in immunohistochemistry - paraffin section to subtype mammary analogue secretory carcinoma and acinic cell carcinoma histologically

Sat Aug 01 00:00:00 UTC 2015

Expression of cellular components in granulomatous inflammatory response in Piaractus mesopotamicus model.

PloS one

Manrique WG,Claudiano Gda S,de Castro MP,Petrillo TR,Pereira Figueiredo MA,Belo MA,Berdeal MI,de Moraes JE,de Moraes FR

PA5-16257 was used in immunohistochemistry - paraffin section to study a Piaractus mesopotamicus model and expression of cellular components in granulomatous inflammatory response

Fri Mar 04 00:00:00 UTC 2016

Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice.

Frontiers in molecular neuroscience

Gombash SE,Cowley CJ,Fitzgerald JA,Hall JC,Mueller C,Christofi FL,Foust KD

PA5-16257 was used in immunohistochemistry - paraffin section to determine the enteric nervous system transduction following systemic AAV9 administration

Fri Oct 31 00:00:00 UTC 2014

MT1-MMP modulates melanoma cell dissemination and metastasis through activation of MMP2 and RAC1.

Pigment cell & melanoma research

Shaverdashvili K,Wong P,Ma J,Zhang K,Osman I,Bedogni B

PA5-16257 was used in immunohistochemistry to study the role of MMP-2 and RAC1 activation in the mechanism by which MT1-MMP promotes melanoma cell motility and metastasis

Sat Mar 01 00:00:00 UTC 2014

A novel electrospun nerve conduit enhanced by carbon nanotubes for peripheral nerve regeneration.

Nanotechnology

Yu W,Jiang X,Cai M,Zhao W,Ye D,Zhou Y,Zhu C,Zhang X,Lu X,Zhang Z

PA5-16257 was used in immunocytochemistry to develop a novel nerve conduit incorporating carbon nanotudes for use in the regeneration of peripheral nerves

Fri Apr 25 00:00:00 UTC 2014

Cortical hypoplasia and ventriculomegaly of p73-deficient mice: Developmental and adult analysis.

The Journal of comparative neurology

Medina-Bolívar C,González-Arnay E,Talos F,González-Gómez M,Moll UM,Meyer G

PA5-16257 was used in immunohistochemistry to study the pathophysiology of two main phenotypic variants of murine brain p73 deficiency

Fri Aug 01 00:00:00 UTC 2014

Low-copy piggyBac transposon mutagenesis in mice identifies genes driving melanoma.

Proceedings of the National Academy of Sciences of the United States of America

Ni TK,Landrette SF,Bjornson RD,Bosenberg MW,Xu T

PA5-16257 was used in immunohistochemistry to identify key melonoma genes using a piggyBac transposon mutagenesis approach in a murine model

Tue Sep 17 00:00:00 UTC 2013

Cutaneous innervation of the human face as assessed by skin biopsy.

Journal of anatomy

Nolano M,Provitera V,Caporaso G,Stancanelli A,Leandri M,Biasiotta A,Cruccu G,Santoro L,Truini A

PA5-16257 was used in immunohistochemistry to use skin biopsy to study human facial cutaneous innervation

Fri Feb 01 00:00:00 UTC 2013

Expression of insulin-like growth factor binding proteins during mouse cochlear development.

Developmental dynamics : an official publication of the American Association of Anatomists

Okano T,Kelley MW

PA5-16257 was used in immunohistochemistry to study the expression of IGFBP family members during development of the murine cochlear

Tue Oct 01 00:00:00 UTC 2013

Role of the neuroglia of human ocular transparent structures in the visual perception concepts.

Bulletin of experimental biology and medicine

Reva GV,Kovaleva IV,Reva IV,Yamamoto T,Novikov AS,Lomakin AV,Kulikova ES

PA5-16257 was used in immunohistochemistry to study the role of human eye neuroglial cells in the perception of vision

Fri Feb 01 00:00:00 UTC 2013

A microscale neuron and Schwann cell coculture model for increasing detection sensitivity of botulinum neurotoxin type A.

Toxicological sciences : an official journal of the Society of Toxicology

Hong WS,Young EW,Tepp WH,Johnson EA,Beebe DJ

PA5-16257 was used in immunocytochemistry to develop a novel bioasay for the highly sensitive detection of botulinum toxin type A based on the co-culture of neurons and Schwann cells on a microscale

Mon Jul 01 00:00:00 UTC 2013

SPARC-like 1 (SC1) is a diversely expressed and developmentally regulated matricellular protein that does not compensate for the absence of SPARC in the CNS.

The Journal of comparative neurology

Lloyd-Burton S,Roskams AJ

PA5-16257 was used in immunohistochemistry to study the expression and role of SPARC-like 1 in the developing and adult nervous system

Wed Aug 15 00:00:00 UTC 2012

Veil nerve-sparing technique and postoperative urinary continence in open antegrade radical prostatectomy.

Urologia internationalis

Shigemura K,Yamanaka N,Yamashita M

PA5-16257 was used in immunohistochemistry to describe a novel technique for open antegrade radical prostatectomy

Thu Apr 25 00:00:00 UTC 2013

A case of xanthoma in a Saanen goat.

Veterinary dermatology

Ozmen O,Haligur M

PA5-16257 was used in immunohistochemistry to report on a case of a Saanen goat with xanthoma

Sun Apr 01 00:00:00 UTC 2012

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