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Invitrogen
RSK3 Polyclonal Antibody
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FIGURE: 1 / 2
RSK3 Antibody (PA5-19697) in ICC/IF
Immunofluorescent staining of HeLa cells using PA5-19697, anti-RSK3 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with PBS-T (20mins), BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain ... View More
Product Details
PA5-19697
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RSK3.
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
PA5-19697 targets RSK3 in IF and WB applications and shows reactivity with Human and mouse samples.
The PA5-19697 immunogen is synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RSK3.
PA5-19697 detects RSK3 which has a predicted molecular weight of approximately 110 kDa.
Target Information
RPS6KA2 (RSK3) serine/threonine kinase is a member of the ribosomal S6 kinase (RSK) family. It is believed that RPS6KA2 is a signal-transducing intermediate in the cellular response to several growth factors. It is found in many tissues, but in higher levels in lung and skeletal muscle. The p90 ribosomal S6 kinases (RSK)1-4 are downstream members of the extracellular signal-regulated kinase (ERK)/MAPK cascade. The loss of RSK2 activity in humans leads to Coffin-Lowry syndrome, which is characterized by mental retardation and growth deficits and may have a tonic regulation on G-protein coupled signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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