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Invitrogen
RNF2 Polyclonal Antibody
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FIGURE: 1 / 1
RNF2 Antibody (PA1-31795) in WB
Western blot detection of RING1B in 20µg NIH-3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Samples were run on a SDS-PAGE, transferred onto nitrocellulose, and probed using a RING1B polyclonal antibody (Product # PA1-31795) at a dilution of 1:500 following incubation at room temperature. Signal was detected using standard techniques.
Product Details
PA1-31795
Product Specifications
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide EAGPSNKRTKTSDC, corresponding to amino acids 189-201 of Human RING1B.
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2253872
Product Specific Information
PA1-31795 detects RING1B from human samples.
PA1-31795 has been successfully used in Western blot applications.
The PA1-31795 immunogen is: Synthetic peptide: EAGPSNKRTKTSDC, corresponding to amino acids 189-201 of Human RING1B.
Target Information
RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). Therefore, it plays a central role in histone code and gene regulation. The ligase activity is enhanced by BMI1/PCGF4. H2AK119Ub gives a specific tag for epigeneti transcriptional repression and participates in X chromosome inactivation of female mammals. RNF2 may be involved in the initiation of both imprinted and random X inactivation. RNF2 is also an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex. This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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