Invitrogen

RFC3 Polyclonal Antibody

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RFC3 Antibody (PA1-27673)

RFC3 knockdown impairs the G1/S cell cycle progression. KG1 cells were transduced with pLKO.1 lentiviral vectors expressing RFC3 shRNA or luciferase shRNA, and then transduced cells were selected with puromycin. (a) Suppressed expression of RFC3 by corresponding specific shRNA was assessed at protein level. Total cell lysates were analyzed by immunoblotting for RFC3. ?-tubulin was used as a loading control.... View More

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RFC3 Antibody — RFC3 knockdown impairs the G1/S cell cycle progression. KG1 cells were transduced with pLKO.1 lentiviral vectors expressing RFC3 shRNA or luciferase shRNA, and then transduced cells were selected with puromycin. (a) Suppressed expression of RFC3 by corresponding specific shRNA was assessed at protein level. Total cell lysates were analyzed by immunoblotting for RFC3. ?-tubulin was used as a loading control. (b) RFC3 knockdown inhibited proliferation of KG1 cells. A total of 1 105 cells were seeded in 12-well plates and the number of viable cells was counted for 3 days. Values represent mean SEM (n = 3). **, p < .01. (c) Cell cycle profile of control or RFC3 knockdown KG1 cells released from thymidine block. Cells were synchronized at G1/S boundary with thymidine treatment (2 mM, 30 h), and then harvested at the indicated times after release into medium with nocodazole (300 nM). Cells were stained with PI following fixation with 70% cold ethanol, and then analyzed for DNA contents by flow cytometry. These data are representative plots from three experiments with similar results. (d) % cell populations at each cell cycle phase were calculated using FlowJo software and denoted as mean SEM (n = 3). {KD}

Product Details

PA1-27673

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 1 publication 1 publication

ELISA (ELISA)

5 µg/mL

Product Specifications

Published species

Human

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to human Replication factor C subunit 3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2178474

Product Specific Information

PA1-27673 detects RFC3 from human and rat samples.

PA1-27673 may be successfully used in ELISA and Western blot applications. By Western blot, this antibody has only been tested on fusion protein.

The immunogen is a synthetic peptide corresponding to human Replication factor C subunit 3.

Target Information

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Replication factor C3 is a CREB target gene that regulates cell cycle progression through the modulation of chromatin loading of PCNA.

Leukemia

Chae HD,Mitton B,Lacayo NJ,Sakamoto KM

PA1-27673 was used in western blot to study CREB in acute myeloid leukemia

Mon Jun 01 00:00:00 UTC 2015

Performance Guarantee

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