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Invitrogen
RAR gamma-2 Polyclonal Antibody
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FIGURE: 1 / 1
RAR gamma-2 Antibody (PA3-814) in WB
Western blot analysis of Retinoic Acid Receptor gamma-2 was performed by loading 25 µg of SK-BR-3 (lane 1) and Hela (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Retinoic Acid Receptor gamma-2 polyclonal antibody (Product # PA3-814) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed usin... View More
Product Details
PA3-814
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues R(13) R L Y G A A G P G A G L L R R(28) of the N-terminal sequence of RAR gamma-2.
Conjugate
Form
Liquid
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2175540
Product Specific Information
PA3-814 detects recombinant human retinoic acid receptor (RAR) gamma 2. The immunizing sequence is 100% conserved in mouse. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR.
PA3-814 has been successfully used in WB, immunoprecipitation and gel shift procedures. In super shift assays this antibody incompletely shifts human RAR gamma-2/retinoid X receptor (RXR)/RAR response element complexes using various synthetic and natural RARE's.
PA3-814 immunizing peptide corresponds to the N-terminus of RAR gamma-2. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR. This peptide (Cat. # PEP-229) is available for use in neutralization and control experiments.
Target Information
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD.||The corresponding gene for the retinoic acid receptor gamma 2 is NR1B3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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