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Invitrogen
Phycoerythrin B Polyclonal Antibody
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FIGURE: 1 / 1
Phycoerythrin B Antibody (PA1-28741) in WB
Western Blot analysis of Phycoerythrin B was performed by loading 1 µg per lane. Lane 1: B-Phycoerythrin reduced. Lane 2: B-Phycoerythrin reduced. Primary antibody: Phycoerythrin B Polyclonal Antibody (Product # PA1-28741) at 1:1,000 for overnight at 4°C. Secondary antibody: Donkey anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT.
Product Details
PA1-28741
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
B-Phycoerythrin collected from Porphyridium cruentum.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS, pH 7.2, with 0.15M NaCl
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1958709
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Phycoerythrin B (PEB) is a type of phycobiliprotein found in certain algae, including red algae and cyanobacteria. It is a fluorescent protein that functions as an accessory pigment in the light-harvesting complexes of photosynthetic organisms. Phycoerythrin B absorbs light primarily in the green-yellow range (approximately 480-580 nm) and emits red fluorescence around 580-700 nm. This pigment is highly efficient in capturing light energy and transferring it to chlorophylls during photosynthesis, thereby enhancing the efficiency of the photosynthetic process, especially underwater where light penetration is limited and green light is more prevalent. PEB's unique optical properties make it a valuable tool in biotechnology applications, such as fluorescent labeling and flow cytometry. The protein is composed of alpha and beta subunits that form hexameric structures, each subunit non-covalently binding linear tetrapyrrole chromophores called phycobilins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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