Invitrogen

Phosphotyrosine Monoclonal Antibody (pY20), AP

Phosphotyrosine Antibody in Western Blot (WB)

FIGURE: 1 / 2

Phosphotyrosine Antibody (03-7722) in WB

Angiogenesis 2010 - Fig. 3 NVP-BHG712 inhibits endogenous EphB4 autophosphorylation in lung tissue of mice and has micromolar exposure in plasma and tissues. NVP-BHG712 has been dosed orally in mice (50 mg/kg). Mice have been sacrificed at time points after oral compound administration as indicated. a To monitor NVP-BHG712 concentration and EphB4 autophosphorylation in lung tissue, one lung lobe were lysed for western analysis... View More

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Product Details

03-7722

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

pY20

Immunogen

Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine

Conjugate

AP AP AP

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

TBS with 40% glycerol, 1mM MgCl2, 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532961

Product Specific Information

This antibody is known to show reactivity towards phosphothreonine.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polyclonal Secondary FGFR2 Mutations Drive Acquired Resistance to FGFR Inhibition in Patients with FGFR2 Fusion-Positive Cholangiocarcinoma.

Cancer discovery

Goyal L,Saha SK,Liu LY,Siravegna G,Leshchiner I,Ahronian LG,Lennerz JK,Vu P,Deshpande V,Kambadakone A,Mussolin B,Reyes S,Henderson L,Sun JE,Van Seventer EE,Gurski JM,Baltschukat S,Schacher-Engstler B,Barys L,Stamm C,Furet P,Ryan DP,Stone JR,Iafrate AJ,Getz G,Porta DG,Tiedt R,Bardelli A,Juric D,Corcoran RB,Bardeesy N,Zhu AX

37722 was used in ELISA to determine the molecular basis for acquired resistance to the fibroblast growth factor receptor inhibitor BGJ398

Wed Mar 01 00:00:00 UTC 2017

Phosphorylation of Tyr245 in the open-inhibited state of Abelson kinase does not induce downstream signaling.

European journal of haematology

Skora L,Kempf D,Mestan J,D'Orazio D,Jahnke W

03-7722 was used in ELISA to investigate the role of Tyr245 phosphorylation in Abelson kinase function

Sun May 01 00:00:00 UTC 2016

NMR reveals the allosteric opening and closing of Abelson tyrosine kinase by ATP-site and myristoyl pocket inhibitors.

Proceedings of the National Academy of Sciences of the United States of America

Skora L,Mestan J,Fabbro D,Jahnke W,Grzesiek S

03-7722 was used in an ELISA assay to analyse the solution conformations of Abelson tyrosine kinase (c-Abl) and c-Abl complexes with ATP-site and allosteric inhibitors.

Tue Nov 19 00:00:00 UTC 2013

The small molecule specific EphB4 kinase inhibitor NVP-BHG712 inhibits VEGF driven angiogenesis.

Angiogenesis

Martiny-Baron G,Holzer P,Billy E,Schnell C,Brueggen J,Ferretti M,Schmiedeberg N,Wood JM,Furet P,Imbach P

03-7722 was used in ELISA to develop and characterize an inhibitor of EphB4 kinase

Wed Sep 01 00:00:00 UTC 2010

The small molecule specific EphB4 kinase inhibitor NVP-BHG712 inhibits VEGF driven angiogenesis.

Angiogenesis

Martiny-Baron G,Holzer P,Billy E,Schnell C,Brueggen J,Ferretti M,Schmiedeberg N,Wood JM,Furet P,Imbach P

03-7722 was used in ELISA to develop and characterize an inhibitor of EphB4 kinase

Wed Sep 01 00:00:00 UTC 2010

Increased Level of Phosphorylated ShcA Measured by Chemiluminescence-Linked Immunoassay Is a Predictor of Good Prognosis in Primary Breast Cancer Expressing Low Levels of Estrogen Receptor.

Cancers

Cicenas J,Küng W,Eppenberger U,Eppenberger-Castori S

03-7722 was used in an ELISA assay to study the link between SH2 domain-containing adaptor protein activation, the estrogen receptor, and primary breast cancer prognosis.

Fri Mar 12 00:00:00 UTC 2010

Evaluation of division-arrested cells for cell-based high-throughput screening and profiling.

Journal of biomolecular screening

Digan ME,Pou C,Niu H,Zhang JH

03-7722 was used in an ELISA assay to explore the potential of division-arrested cells as a substitution for dividing cells during cell-based high-throughput screening (HTS).

Thu Sep 01 00:00:00 UTC 2005

In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.

Cancer cell

García-Echeverría C,Pearson MA,Marti A,Meyer T,Mestan J,Zimmermann J,Gao J,Brueggen J,Capraro HG,Cozens R,Evans DB,Fabbro D,Furet P,Porta DG,Liebetanz J,Martiny-Baron G,Ruetz S,Hofmann F

Mon Mar 01 00:00:00 UTC 2004

AEE788: a dual family epidermal growth factor receptor/ErbB2 and vascular endothelial growth factor receptor tyrosine kinase inhibitor with antitumor and antiangiogenic activity.

Cancer research

Traxler P,Allegrini PR,Brandt R,Brueggen J,Cozens R,Fabbro D,Grosios K,Lane HA,McSheehy P,Mestan J,Meyer T,Tang C,Wartmann M,Wood J,Caravatti G

Thu Jul 15 00:00:00 UTC 2004