Invitrogen

Phospho-p53 (Ser20) Polyclonal Antibody

Phospho-p53 (Ser20) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-p53 (Ser20) Antibody (PA5-17894) in ICC/IF

Immunofluorescence analysis of Phospho-p53 (Ser20) was performed using 70% confluent log phase HCT116 cells treated with etoposide. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p53(Ser20) Polyclonal Antibody (Product # PA5-17894) at 1:100 dilution... View More

Phospho-p53 (Ser20) Antibody in Western Blot (WB)

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Product Details

PA5-17894

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer20 of human p53

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

33 µg/mL

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10981312

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with p53 phosphorylated at other sites.

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses.

mBio

Mertens ME,Knipe DM

PA5-17894 was used in Western Blot to show that the DDR kinase pathways activated in normal human fibroblasts by herpes simplex virus 1 (HSV-1) input genomic DNA, HSV-1 replicating DNA, and progeny DNA and in uninfected cells treated with etoposide are different.

Tue Feb 09 00:00:00 UTC 2021