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Invitrogen
Phospho-c-Raf (Ser621) Polyclonal Antibody
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FIGURE: 1 / 8
Phospho-c-Raf (Ser621) Antibody (PA1-26655) in WB
Western blot antibody-peptide competition and mutant analysis using a Phospho-c-Raf pSer621 polyclonal antibody (Product # PA1-26655). Immunoprecipitates of HEK293 cells overexpressing wild-type c-Raf and stimulated with 50 ng/ml EGF for 3 minutes (1-4) or c-Raf mutant S621A (5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer ov... View More
Product Details
PA1-26655
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 621. The sequence is conserved in mouse and rat.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_779919
Product Specific Information
PA1-26655 detects c-Raf (phospho S621) from human samples. PA1-26655 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology.
PA1-26655 has been successfully used in Western blot procedures.
The PA1-26655 immunogen is a synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 621. The sequence is conserved in mouse and rat.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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