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Invitrogen
Phospho-IRE1 alpha (Ser724) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-IRE1 alpha (Ser724) Antibody (PA5-85647) in WB
Western Blot was performed using Anti-Phospho-IRE1 alpha (Ser724) Polyclonal Antibody (Product # PA5-85647) and a 110 kDa band corresponding to Serine/threonine-protein kinase/endoribonuclease IRE1 was observed in HeLa cells treated with EGF. Membrane enriched extracts (60 µg lysate) of HeLa (Lane 1) and HeLa treated with 100 ng/mL of EGF for 20 mins (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tri... View More
Product Details
PA5-85647
Applications
Tested Dilution
Publications
Product Specifications
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IRE1.
Conjugate
Form
Liquid
Concentration
0.84 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792787
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (100%).
Positive Control: 293T, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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