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Invitrogen
Phospho-IKK alpha/beta (Ser176, Ser177) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-IKK alpha/beta (Ser176, Ser177) Antibody (PA5-35835) in WB
Western blot analysis of Phospho-IKK alpha pSer176+IKK-beta pSer177 in TNF-treated NIH-3T3 whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-IKK alpha pSer176+IKK-beta pSer177 polyclonal antibody (Product # PA5-35835).
Product Details
PA5-35835
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from human IKK- alpha /IKK- beta around the phosphorylation site of Serine 177
Conjugate
Form
Liquid
Concentration
Lot-Specific
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2553145
Product Specific Information
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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