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Invitrogen
Phospho-GSK3 alpha/beta (Tyr279 , Tyr216) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-GSK3 alpha/beta (Tyr279 , Tyr216) Antibody (PA5-35482) in WB
Western blot analysis of Phospho-GSK3 alpha pTyr279+GSK3 beta pTyr216 in HeLa whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-GSK3 alpha pTyr279+GSK3 beta pTyr216 polyclonal antibody (Product # PA5-35482).
Product Details
PA5-35482
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GSK3 alpha/ beta around the phosphorylation site of Tyrosine 279/216
Conjugate
Form
Liquid
Concentration
Lot-Specific
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2552792
Product Specific Information
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Target Information
Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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