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Invitrogen
Phospho-CD247 (CD3 zeta) (Tyr142) Polyclonal Antibody
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Phospho-CD247 (CD3 zeta) (Tyr142) Antibody (PA5-118592) in ICC/IF
Immunocytochemical analysis of Phospho-CD247 (CD3 zeta) (Tyr142) in Hela cells using a Phospho-CD247 (CD3 zeta) (Tyr142) polyclonal antibody (Product # PA5-118592). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated go... View More
Product Details
PA5-118592
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2903092
Target Information
CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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