Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phosphatidylinositol 3,4,5-trisphosphate Monoclonal Antibody (RC6F8)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 2
Phosphatidylinositol 3,4,5-trisphosphate Antibody (A-21328) in ICC/IF
Localization of phosphatidylinositol 3, 4, 5-triphosphate in unstimulated (left panel) and insulin-stimulated (right panel) 3T3-L1 cells detected using anti-phosphatidylinositol 3, 4, 5-triphosphate, mouse IgM monoclonal RC6F8 (Product # A-21328) and visualized using biotinylated goat anti-mouse IgM in conjunction with Alexa Fluor® 488 streptavidin (Product # S-11223). Image courtesy of Echelon Research Lab... View More
Product Details
A-21328
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Mouse
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
RC6F8
Immunogen
phosphatidylinositol 3,4,5-diphosphate
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.2, with 0.1% BSA
Contains
5mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2535837
Target Information
Phosphatidylinositol (3, 4, 5)-trisphosphate (PtdIns(3, 4, 5)P3) commonly abbreviated to PIP3 is the product of the class I phosphoinositide 3-kinases (PI 3-kinases) activity on phosphatidylinositol (4, 5)-bisphosphate. PtdIns (3, 4, 5) P3 is dephophosphorylated by the phosphatase, PTEN on the 3 position and by SHIPs (SH2-containing inositol phosphatase) on the 5' position of the inositol ring. The PH domain in a number of proteins binds to PtdIns (3, 4, 5) P3. Such proteins include Akt/PKB, PDK1, Btk1 and ARNO. The generation of PtdIns (3, 4, 5) P3 at the plasma membrane upon the activation of class I PI 3-kinases causes these proteins to translocate to the plasma membrane and accordingly affects their activity. The PH domain allows binding between PtdIns (3, 4, 5) P3 and G-protein coupled receptor kinases (GRKs). This enhances the binding of the GRK to the plasma membrane. [National Center for Biotechnology Information. PubChem Compound Database; CID=53477782].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support