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Invitrogen
Phl p 1 Chimeric Recombinant Rabbit Monoclonal Antibody (Clone 25)
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FIGURE: 1 / 1
Phl p 1 Chimeric Antibody (MA5-48033) in ELISA
ELISA analysis of Phl p 1 chimeric monoclonal antibody (Product # MA5-48033) was performed by coating plate with Phl p 1 protein at a concentration of 2 µg/mL. A 3-fold serial dilution from 10,000 to 0.1 ng/mL of the antibody was done. For detection, a 1:4,000 dilution of HRP-labelled goat anti-rabbit antibody was used.
Product Details
MA5-48033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
Clone 25
Immunogen
Recombinant Phl p 1, a grass pollen allergen.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074476
Product Specific Information
Specificity: This antibody binds to the Timothy grass allergen Phl p 1 and shows cotss reactivity with natural group 1 allergens from a variety of grass species such as oat, sweet vernal grass, Bermuda grass, rye grass, common reed, Kentucky blue grass, rye, wheat and maize (weak).
Target Information
Phl p 1 binds to specific receptors on the surface of immune cells called TLR4 (Toll-like receptor 4) and MD-2 (myeloid differentiation factor 2). This binding triggers a signaling cascade that leads to the activation of immune cells and the release of inflammatory mediators, which cause the allergic symptoms. The interaction between Phl p 1 and TLR4-MD-2 complex has been studied extensively and is considered a key event in the development of grass pollen allergy. Understanding the molecular mechanisms involved in this interaction is important for developing new therapeutic strategies to treat or prevent allergic reactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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