Invitrogen

PSMC5 Polyclonal Antibody

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PSMC5 Antibody (PA1-970) in WB

Western blot of proteasome 19S subunit S8 on HeLa cell lysate using Product # PA1-970.

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PSMC5 Antibody — IP-MS enrichment of PSMC5 (LFQ intensity): PSMC5 was enriched 297-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSMC5 antibody (Product # PA1-970). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

PA1-970

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human recombinant proteasome 19S subunit S8.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2172669

Product Specific Information

PA1-970 detects proteasome 19S subunit S8 from human cells.

PA1-970 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45 kDa protein representing proteasome 19S subunit S8 from HeLa cell lysate.

PA1-970 antigen is recombinant proteasome 19S subunit S8.

Target Information

Human SUG1 (also known as p45, Rpt6 and Cim3) is a component of the 19S regulatory subunit of the 26S proteosome complex. It is one of the AAA ATPas found in the 19S regulatory complex that is thought to participate in the unfolding of ubiquitinated proteins in an ATP-dependent manner. It has also been shown to directly interact with the nucleotide excision repair protein XPB.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response.

The Journal of experimental medicine

Saccani S,Marazzi I,Beg AA,Natoli G

PA1-970 was used in chromatin immunoprecipitation to investigate the effect of promoter-bound p65/RelA degradation on the nuclear factor kappaB response termination.

Mon Jul 05 00:00:00 UTC 2004

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