Invitrogen

PPARGC1A Polyclonal Antibody

PPARGC1A Antibody in Immunocytochemistry (ICC/IF)

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PPARGC1A Antibody (PA5-38022) in ICC/IF

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling PPARGC1A with PGC1 alpha Polyclonal Antibody (Product # PA5-38022) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

PPARGC1A Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-38022

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

17 amino acid peptide near the amino terminus of human PPARGC1A

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Wet ice

AB_2554626

Product Specific Information

A recommended positive control is human heart tissue lysate.

At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms.

Target Information

PGC1 alpha is a transcriptional coactivator that regulates the genes involved in energy metabolism. It interacts with PPARgamma, which permits it's interaction with multiple transcription factors. PGC-1 Alpha can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. It may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.

International journal of molecular sciences

Cardile A,Zanrè V,Campagnari R,Asson F,Addo SS,Orlandi E,Menegazzi M

PA5-38022 was used in Immunohistochemistry (Paraffin), Western Blot to provide evidence of the pleiotropic antitumor effects induced by HPF in melanoma cells.

Mon Jan 09 00:00:00 UTC 2023

The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.

Nature communications

Lee H,Lee TJ,Galloway CA,Zhi W,Xiao W,de Mesy Bentley KL,Sharma A,Teng Y,Sesaki H,Yoon Y

PA5-38022 was used in Western Blot to demonstrate that OPA1 is dispensable in the liver, and that the mitohormesis induced by OPA1 LKO prevents liver injury and contributes to liver resiliency.

Mon Oct 23 00:00:00 UTC 2023

An expanded population of CD8^dim T cells with features of mitochondrial dysfunction and senescence is associated with persistent HIV-associated Kaposi's sarcoma under ART.

Frontiers in cell and developmental biology

Clutton GT,Weideman AMK,Goonetilleke NP,Maurer T

PA5-38022 was used in Flow Cytometry to indicate that an expanded CD8dim T cell population displaying features of senescence and mitochondrial dysfunction is associated with KS disease under ART.

Thu Jun 01 00:00:00 UTC 2023

Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system.

Journal of advanced research

Su H,Liu N,Zhang Y,Kong J

PA5-38022 was used in Western Blot to study the potential role of vitamin D in energy homeostasis in the CNS and elaborate its underlying mechanisms.

Mon Nov 01 00:00:00 UTC 2021

Nilotinib Improves Bioenergetic Profiling in Brain Astroglia in the 3xTg Mouse Model of Alzheimer's Disease.

Aging and disease

Adlimoghaddam A,Odero GG,Glazner G,Turner RS,Albensi BC

PA5-38022 was used in Immunohistochemistry (Paraffin), Western Blot to support a role for nilotinib in improving mitochondrial function and suggest that astroglia may be a key therapeutic target in treating AD.

Thu Apr 01 00:00:00 UTC 2021

Nilotinib Improves Bioenergetic Profiling in Brain Astroglia in the 3xTg Mouse Model of Alzheimer's Disease.

Aging and disease

Adlimoghaddam A,Odero GG,Glazner G,Turner RS,Albensi BC

Thu Apr 01 00:00:00 UTC 2021

Nutmeg extract potentially alters characteristics of white adipose tissue in rats.

Veterinary medicine and science

Lesmana R,Siannoto M,Nugraha GI,Goenawan H,Feinisa AK,Pratiwi YS,Veronica F,Tarawan VM,Susianti S,Supratman U

Published figure using PGC1 alpha polyclonal antibody (Product # PA5-38022) in Western Blot

Mon Mar 01 00:00:00 UTC 2021

HDAC4 Knockdown Alleviates Denervation-Induced Muscle Atrophy by Inhibiting Myogenin-Dependent Atrogene Activation.

Frontiers in cellular neuroscience

Ma W,Cai Y,Shen Y,Chen X,Zhang L,Ji Y,Chen Z,Zhu J,Yang X,Sun H

PA5-38022 was used in Western Blotting to find that the expression of HDAC4 increased significantly in denervated skeletal muscle.

Tue Jul 20 00:00:00 UTC 2021

HDAC4 Knockdown Alleviates Denervation-Induced Muscle Atrophy by Inhibiting Myogenin-Dependent Atrogene Activation.

Frontiers in cellular neuroscience

Ma W,Cai Y,Shen Y,Chen X,Zhang L,Ji Y,Chen Z,Zhu J,Yang X,Sun H

PA5-38022 was used in Western Blotting to find that the expression of HDAC4 increased significantly in denervated skeletal muscle.

Tue Jul 20 00:00:00 UTC 2021

Renal Glomerular Mitochondria Function in Salt-Sensitive Hypertension.

Frontiers in physiology

Domondon M,Polina I,Nikiforova AB,Sultanova RF,Kruger C,Vasileva VY,Fomin MV,Beeson GC,Nieminen AL,Smythe N,Maldonado EN,Stadler K,Ilatovskaya DV

PA5-38022 was used in Western Blot to report that glomerular mitochondria in SS hypertension are functionally and structurally defective, and this impairment could eventually lead to loss of podocytes and proteinuria.

Mon Sep 28 00:00:00 UTC 2020

CITED2 mediates the mechanical loading-induced suppression of adipokines in the infrapatellar fat pad.

Annals of the New York Academy of Sciences

Liu L,He Z,Xu L,Lu L,Feng H,Leong DJ,Kim SJ,Hirsh DM,Majeska RJ,Goldring MB,Cobelli NJ,Sun HB

PA5-38022 was used in Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry to suggest that CITED2 is a critical regulator in physiologic loading-induced chondroprotection in the context of a high-fat diet and PGC-1a is required for the inhibitory effects of CITED2 on the expression of adipokines in the infrapatellar fat pad.

Mon Apr 01 00:00:00 UTC 2019

Increased Frataxin Expression Induced in Friedreich Ataxia Cells by Platinum TALE-VP64s or Platinum TALE-SunTag.

Molecular therapy. Nucleic acids

Cherif K,Gérard C,Rousseau J,Ouellet DL,Chapdelaine P,Tremblay JP

PA5-38022 was used in Western Blotting to show the aconitase activity was positively modulated by the frataxin level in mitochondria, and it was, thus, increased in vitro and in vivo by the increased frataxin expression.

Fri Sep 07 00:00:00 UTC 2018

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