Invitrogen

PMEL Polyclonal Antibody

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PMEL Antibody (PA5-32491) in WB

Western blot was performed using Anti-PMEL Polyclonal Antibody (Product # PA5-32491) and an 80 kDa band corresponding to PMEL was observed across in SK-MEL-5 in comparison to A-375 and SK-BR-3 which is supposed to be negative. A non-specific band around 58 kDa was also observed. Whole cell extracts (30 µg lysate) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB230... View More

PMEL Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PMEL Antibody — Figure 7 Melanoma cell response to vemurafenib treatment in the 3D microenvironment. ( A - D ) Evaluation of melanoma cell proliferation in the model after 12 days of treatment with vemurafenib. Ki67 immunostaining (green signal) is indicative of cell proliferation in the WM983B melanoma tumor ( B , D , red signal, PMEL). ( E ) Proliferative index of WM983A and WM983B cells in the 3D model in response to increasing vemurafenib concentrations. ( F - I ) Co-labeling of apoptotic cells after staining for cleaved caspase 3 and tumor marker NG2. ( F , H ) Immunostaining for cleaved caspase 3 (red signal) in the melanoma model with ( G , I ) immunostaining of melanoma cells (neural/glial antigen 2 (NG-2), green signal and Hoechst, blue signal). ( J ) Quantification of cleaved caspase-3 expressing cells per total number of tumor cells as a function of vemurafenib concentration. (*p <= 0.05 as determined by ANOVA followed by a multiple comparison). Results are representative of two independent experiments, performed in triplicate. {TM}

Product Details

PA5-32491

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encoding aa 1-150 human gp100.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.123 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2549958

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is melanoma.

Target Information

Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development.

Scientific reports

Bourland J,Fradette J,Auger FA

Published figure using PMEL polyclonal antibody (Product # PA5-32491) in Immunofluorescence

Tue Sep 04 00:00:00 UTC 2018

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