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Invitrogen
PHRF1 Polyclonal Antibody
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FIGURE: 1 / 1
PHRF1 Antibody (PA5-116730) in WB
Western blot analysis of PHRF1 in extracts of various cell lines. Samples were incubated with PHRF1 Polyclonal antibody (Product # PA5-116730) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-116730
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1350-1450 of human PHRF1 (NP_0012735121)
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901361
Product Specific Information
Positive Samples: Juakat, K-562, HepG2, HeLa
Immunogen sequence: KAEAPSSPDV APAGKEDSPS ASGRVQEAAR PEEVVSQTPL LRSRALVKRV TWNLQESESS APAEDRAPRA PLHRPQKPRE GAWDMEDVAP TGVRQVFSEL P
Target Information
Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTD-binding SR-like protein rA9; PHD and RING finger domain-containing protein 1; protein phosphatase 1, regulatory subunit 125
Gene Aliases: KIAA1542; PHRF1; PPP1R125; RNF221
UniProt ID: (Human) Q9P1Y6
Entrez Gene ID: (Human) 57661
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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