Invitrogen

PGAM2 Polyclonal Antibody

PGAM2 Antibody in Immunohistochemistry (IHC)

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PGAM2 Antibody (PA5-24006) in IHC

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human skeletal muscle using a PGAM2 polyclonal antibody (Product # PA5-24006), followed by HRP-conjugated secondary antibody and DAB staining.

Product Image Gallery

PGAM2 Antibody — Fig 5 Western blots of markers for apoptosis, autophagy, fatty acid metabolism, and glycolysis in left ventricular homogenates of control, IgG-Fc-treated, and ephrinA1-Fc-treated mouse hearts at 24hrs. (a)The ratio of bcl2/bax increased 2-fold and HSP20 increased by 35%, both of which are indicative of reduced apoptosis in ephrinA1-Fc treated mouse hearts compared to IgG-Fc. (b) The ratios of LC3II/LC3I increased 28% and pmTOR/mTOR decreased by 31%, indicating increased autophagy in ephrinA1-Fc relative to IgG-Fc-treated mouse hearts. (c) Decreased CD36 and MCD in ephrinA1-Fc-treated mice by 35% and 70% respectively compared to IgG-Fc treated mice is suggestive of reduced deleterious fatty acid accumulation and increased (d) PGAM2 and PDK2 by 26% and 33% respectively indicate altered glycolytic flux. *p<0.05 compared to control, ** p<0.05 compared to IgG-Fc, + p<0.01 compared to control, ++ p<0.01 compared to IgG = Fc, ++ p<0.001 compared to control, ++++ p<0.001 compared to IgG-Fc. {TM}

Product Details

PA5-24006

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human PGAM2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Lot-specific

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2541506

Target Information

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM2 encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EphrinA1-Fc attenuates myocardial ischemia/reperfusion injury in mice.

PloS one

DuSablon A,Parks J,Whitehurst K,Estes H,Chase R,Vlahos E,Sharma U,Wert D,Virag J

Published figure using PGAM2 polyclonal antibody (Product # PA5-24006) in Western Blot

Tue Jan 16 00:00:00 UTC 2018

Performance Guarantee

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